In double-quenched BHQnova Probes the nova quencher is closer to the fluorophore which serves to enchance quenching efficacy. A higher quenching efficiency lowers background fluorescence and ...
These dual-labeled hydrolysis probes consist of a 5' fluorescent reporter dye and a 3' Eclipse Dark Quencher (EDQ) coupled to the minor groove binder (MGB) moiety. Image Credit: LGC Biosearch ...
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