In double-quenched BHQnova Probes the nova quencher is closer to the fluorophore which serves to enchance quenching efficacy. A higher quenching efficiency lowers background fluorescence and ...
BHQ TM Probes are traditional, linear, dual-labeled FRET probes with a fluorophore and a quencher covalently linked to the 5’ and 3’ ends, respectively. They are typically 20 to 30 bases in ...